Essays /

Cancer Patients Reject Treatment Essay

Essay preview

Lecture 2: DNA Replication, PCR, Sequencing; Chapter 7
DNA is composed of nucleotides: purines and pyrimidines.
These are nucleotides with either two rings or a single one. The difference is essential to the hydrogen bonding. The structure allows specificity of the structure. A-T and G-C. Specificity is based on how thse molecules are shaped and their ability on making hydrogen bonds. A single hydrogen bond is low energy bond. Many and thousands of hydrogen bonds summoned will become like Velcro which can be extremely strong. NUCLEOTIDES CAN BE POLYMERIZED.

How can you make a polynucleotide?
Dehydration reaction: Remove a water molecule to get subunits. DNA chain has a 5 and 3 prime end. Refers to different carbons on the deoxyribose. 5 prime has a phosphate on it and 3 prime end has a hydroxyl group on it. The way you polymerize the DNA is by taking a precursor nucleotide triphosphate. It has 3 phosphates group on its five prime end. These phosphates group interact with the 3 prime hydroxyl. Two of these phosphates are cleaved off and hydrolyzed to form a new phosphodiester bond between them. DNA forms a double helix?

How do you make sure you get the right nucleotide? The way you do that is the properties of DNA and base pairing of nucleotides. This is a structure DNA as a double helix. In the double helix is the two strands of DNA are compleme...

Read more

Keywords

-65 /webcontent/anisamples/molecularbiology/pcr.html 1o 2 2nd 3 5 50 7 72 80 9 90 95 a-t abil act ad add agent ahead allow along alow amplifi anneal anoth antiparallel automat back bacteri bacteria base becom begin bidirect bind bit bond break bubbl c call cancer carbon caus cell centigrad chain chapter chromosom cipro cleav coat complementari complet complex compon compos consist continu copi coval cover cursor cut cycl death definit degre dehydr denatur deoxyribos differ direct dna dntps doubl duplic either end ene energi enzym error essenti eukaryot eventu everi excis exonucleas expos extend extrem fall final find five flip fork form fragment front g g-c gap general genom get go gone group gyras happen heat held helicas helix human hydrogen hydrolyz hydroxyl import incorpor individu inhibit inhibitor interact invert involv join know lag lead lectur left length ligas ligat like link littl long look lot low made make mani microscop mistak model molecul move multipl necessari need new nucleotid occur okazaki oligonucleotid one open opposit optimum order orient origin pair parent patient pcr pharmaceut phosphat phosphodiest place pol polymer polymeras polynucleotid precursor prescrib prevent primas prime primer process proofread properti protein purin pyrimidin quick radioact rais re reaction realli reduc refer region reject rejoin relat reliev remov repeat replic requir right ring rna scratch seal second see semiconserv separ sequenc serv shape short show singl slow small special specif specifi spread ssb ssbs start step strand strong structur subunit sumanasinc.com sumanasinc.com/webcontent/anisamples/molecularbiology/pcr.html summon sure take taq target temperatur templat tension thermost think thousand thse time togeth toward treatment triphosph twist two unwind unzip velcro water way weak well wouldn wrong youallow zip