Essays /

Candidate Essay

Essay preview

For serial dilutions, you want to start at 0 concentration to autozero the machine. Even if you’re not using the first tube to autozero the machine, you want to know your background reading. For the upper limit, if you suspect one, you might go slightly above that by a few percentage point. For how you pick the values in the middle, you want a “reasonable spread.” If you’re going from 0 to 12, and you want 3 or 4 points in between to get a nice trendline. 5 to 6 points for a good trendline. Gel system in topic 11 doesn’t require denaturing. The buffer system has no reagent to denature the protein, so the separation is based on the net charge. The protein doesn’t change its shape while moving through the cell, it stays in its “native” state. Because we are under native conditions, the separation is based on charge. If we were denaturing the protein, then we would be concerned about molecular weight. What’s the diff. between normal hemoglobin and mutated hemoglobin in sickle cell patients? Normal hemoglobin has two more acidic residues, and in mutat...

Read more

Keywords

0 10 11 12 3 4 5 6 6.8 7 9.2 a.a.s abl absorpt acid affect aldehyd aldos also amino amplifi analyz anneal anod anoth aren assay atp autozero axe axi back background bacteri base basic beer black block blue box bromid bromophenol buffer build cabl calcium calcul candid carbohydr carboxyl care cathod cell certain chain chang charg chlorid chromosom cofactor color compon concentr concern condit connect consist contain contamin control curv cyanol data demonstr denatur design diff differ dilut distribut dna dntps doesn dye e7 e8 easi effici electrophoresi emulsifi end energi environ equat error ethidium even experi exposur fail faster fat figur first follow function gel get give given go good graph group growth harm heat help hemoglobin high higher how/where howev human import includ incubation/recovery individu intercal isomer ketos know label lack last law layer lb lb/amp leftov less limit linear lipid list load log look machin make mani marker max maxim media memor mgcl2 middl might migrat mitochondri mix mixtur molar molecul molecular monitor monom monosaccharid move movement mtdna mutat nativ need negat net new nice normal nucleic nucleotid obtain oil one origin oxid parallel partner patient pcr percentag pglo ph phase phosphat phosphatas pi pick plate point polym polymeras posit possibl practic predict prepar primer principl problem procedur produc product protein pure purpos re reaction read reagent reason recogn red reduc repeat replac report requir residu ring sampl scale second see separ serial serv set shape shock shook show sickl side slight slower small solv sourc specif spectrophotomet spread stain standard start state stay still sucros sudan sugar suspect system tabl taq tell templat three time titl tntc topic transform treatment trendlin tube two type unit unknown unless upper use uv v valu version want water wavelength weight well wild without won would x x-axi xylan μl