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Identifying Micrococcus luteus

Identifying Micrococcus luteus

There are many reasons for establishing the identity of a microorganism. The reasons range from the knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. Laboratory scientists are able to isolate, identify, and determine the antimicrobial susceptibility pattern. Some methods that are used in laboratory settings are: the use of microscopy whether it’s using wet mounts or stains, macroscopic antigen detection, cultures, and susceptibility testing. When using microscopy methods, wet mounts don’t require the use of stains or dyes due to the fact that the microbe is usually large and/or motile. Microbes that aren’t large and/or motile are stained. Without staining, bacteria are difficult to visualize. “Gram staining is the most useful stain procedure, for the fact that it differentiates between organisms with thick peptidoglycan cell walls, which are gram-positive, and those with thin peptidoglycan cell walls and outer membranes that dissolve with alcohol or acetone, these being gram-negative, (Fauci, 2009).” Macroscopic antigen detection is used to identify microbes by means of their protein antigen. Cultures are collected for bacterial isolation; this relies on the use of artificial media that will support bacterial growth, an example being a nutrient agar. Susceptibility testing allows the physician to choose antimicrobial agents and also identify potential ...

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