Identifying Micrococcus luteus

Identifying Micrococcus luteus

Introduction
There are many reasons for establishing the identity of a microorganism. The reasons range from the knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. Laboratory scientists are able to isolate, identify, and determine the antimicrobial susceptibility pattern. Some methods that are used in laboratory settings are: the use of microscopy whether it’s using wet mounts or stains, macroscopic antigen detection, cultures, and susceptibility testing. When using microscopy methods, wet mounts don’t require the use of stains or dyes due to the fact that the microbe is usually large and/or motile. Microbes that aren’t large and/or motile are stained. Without staining, bacteria are difficult to visualize. “Gram staining is the most useful stain procedure, for the fact that it differentiates between organisms with thick peptidoglycan cell walls, which are gram-positive, and those with thin peptidoglycan cell walls and outer membranes that dissolve with alcohol or acetone, these being gram-negative, (Fauci, 2009).” Macroscopic antigen detection is used to identify microbes by means of their protein antigen. Cultures are collected for bacterial isolation; this relies on the use of artificial media that will support bacterial growth, an example being a nutrient agar. Susceptibility testing allows the physician to choose antimicrobial agents and also identify potential ...

1 10 13 17th 1999 20 2008 2009 2011 216 24 333 37 4 abdomin abl aceton acid acquir aerob agar age agent air alcohol alderson allow along also ampicillin and/or anoth antibiot antigen antimicrobi appli aren arthriti articular artifici associ bacteremia bacteri bacteria baltimor beer begin berkow better bi bi-plat biochem blood blue blue/violet breath bright cathet catheter-rel caus causat caviti ceftriaxon cell certain chang characterist chill choos class cluster cocci collect coloni color concentr concern conclud conclus confirm consid contact contain contamin control correct correl could cultur d dark debilit depend describ detect determin diagnosi diagnost diarrhea differ differenti difficult direct discuss diseas dissolv done dramat duben duben-engelkirk duben-engelkrik due dust dye ed endocard engelkirk engelkrik environ essenti establish exampl except experi extra extra-articular fact fail far fatig fauci fever final find first fluid follow food form found fountainhead g gave given go golden grade gram gram-neg gram-posit grow grown growth h hand handwash happen harrison headach health/lippincott high hill histori hospit hour ident identif identifi ill immunocompromis improv incub indigen indirect infect infecti infection-control initi inocul instructor insuffici introduct irregular isol iv j joint kluwer know known l lab label laboratori larg learn line littl loss low luteus m macconkey macroscop main make malais mani mannitol manual materi may mcgraw mcgraw-hil mean media medic medicin medium membran mening merck method microb microbiolog micrococcus microflora microorgan microscopi million moder morpholog motil motion mount mouth msa mucous n.j name nasal nausea neck need negat new night normal nosocomi nutrient object occas often one onto opportunist organ origin outer overal oxygen p pain pair paper past path pathogen patient pattern penicillin peptidoglycan perform person pg physician pigment plate pneumococc pneumonia posit potenti practic preced presenc present press prevent problem procedur prodrom produc proper protein pure puriti purpos r rang rare re reaction reason refer relat reli requir research respiratori respons rest restrict result rich salt scientist second see select septic set sever shake show side sign sinc skill skin slant slat smooth soft soil sore southlak specif spike staff stain station step steril stiff streak studi support suscept sweat symptom tachypnea techniqu temperatur tender test tetrad texa therapi thick thin third thrive throat thus tract transfer transmiss transmit treat u/day under unknown upper use usual valvular vancomycin violet visual vomit wall warmth wash water week weight well wet whether whitehous wilkin william without wolter work yellow york zag zig zig-zag